Graduated master students

Lucie Borková

Borková Lucie Ph.D.'s picture
Master training

Supervisor:
Status: Graduated 2012 to 2014
Modification of the position 2 in betulin, allobetulin and betulinic acid with polar substituents
The main aim of the study is to modify lupane and 18-αoleanane triterpenoids in order to obtain a set of new derivatives with higher cytotoxicity and better solubility than the starting material.

Lucie Broskevičová

Broskevičová Lucie's picture
Master training

Status: Graduated 2014 to 2016
Typing of systemic amyloidosis using mass spectrometry-based proteomic analysis

Tereza Buchtová

Buchtová Tereza's picture
Master training

Supervisor:
Status: Graduated 2016 to 2018
DNA demage responses in BRCA1 KO Sus Srofa cell line

Hana Crlíková

Crlíková Hana's picture
Master training

Supervisor:
Status: Graduated 2015 to 2016
Stabilisation of PCR reagents for storing at room temperature
Vypracováním teoretické části diplomové práce student prokáže schopnost orientovat se v tématech skladování bioreagencií pro PCR, pracovat s odbornými cizojazyčnými texty a řádně citovat. V praktické části student prokáže schopnost samostatně naplánovat, provést a vyhodnotit laboratorní experiment, týkající se PCR, inhibičních řad a testování stability DNA

Monika Cvancigerová

Cvancigerová Monika's picture
Master training

Status: Graduated 2015 to 2017
Genetic factors of sarcoidosis

Veronika Černínová

Černínová Veronika's picture
Master training

Supervisor:
Status: Graduated 2014 to 2016
Preparation of new derivatives of the imidazopyridine with potential CNS activity

Kristýna Dostálová

Dostálová Kristýna's picture
Master training

Supervisor:
Status: Graduated 2014 to 2016
Cell-based assays for high-throughput screening in drug discovery

Martina Drábiková

Drábiková Martina's picture
Master training

Supervisor:
Status: Graduated 2012 to 2014
Synthesis of heterocyclic compound containing a sulfone or sulfonamide

Petra Drábková

Drábková Petra B.Sc.'s picture
Master training

Status: Graduated 2014 to 2016
Solid-phase synthesis of novel tricyclic heterocycles with 4-chloro-2-fluoro-5-nitrobenzoic acid
Master’s thesis is focused on solid-phase preparation of heterocycles with three condensed cycles combined benzodiazepine structure and six or seven member heterocycles using multi-reactive building block 4-chloro-2-fluoro-5-nitrobenzoic acid.

Ivana Družbíková

Družbíková Ivana's picture
Master training

Status: Graduated 2016 to 2018
Strategy of human disease model preparation

Barbara Duchoňová

Duchoňová Barbara's picture
Master training

Status: Graduated 2015 to 2017
Detection of ribosomal stress

Dominika Flašarová

Flašarová Dominika's picture
Master training

Supervisor:
Status: Graduated 2016 to 2018
KRAS signalling pathway in relation to pancreatic ductal adenocarcinoma

Gabriela Fryčová

Fryčová Gabriela's picture
Master training

Status: Graduated 2016 to 2018
Methylome profiling in cancer cells for identification of bevacizumab response biomarkers
Aberrant regulation at epigenetic level can directly and/or indirectly influence the genetic integrity and gene expression pattern of cells, resulting in the development of various types of disorders, including cancer. The local hypermethylation of tumor-suppressor genes and the global hypomethylation of genomic DNA often occur in human cancers. Multiple DNA methylation changes in the cancer methylome could be associated with the acquisition of drug resistance. At our institute we are currently trying to identify new diagnostic biomarkers, which could be potentially used for better treatment personalization by bevacizumab (Avastin). Bevacizumab is a monoclonal antibody against plasma protein VEGF (vascular endothelial growth factor), which has antiangiogenic effects and is used in clinical practice for treatment of the metastatic colorectal carcinoma (CRC). We assume that genome-wide profiling of DNA methylation could help to identify undiscovered changes associated with good or bad answers to the drug and that it could also help to improve diagnostics of CRC.

Gabriela Gabčová

Gabčová Gabriela B.Sc.'s picture
Master training

Supervisor:
Status: Graduated 2010 to 2015
Detection of predictive genetic oncomarkers of EGFR signalling pathway using sequencing technologies
The thesis is reviewing PCR-based methods of mutation detection in NRAS gene while experimental part is focused on pyrosequencing.

Michaela Galoczova

Galoczova Michaela's picture
Master training

Status: Graduated 2013 to 2015
Metabolomic analysis of the urine of newborns with perinatal asphyxia

Klára Habartova

Habartova Klára's picture
Master training

Supervisor:
Status: Graduated 2013 to 2015
Biochemical analysis of joint effusion

Barbora Agatha Halouzková

Halouzková Barbora Agatha's picture
Master training

Supervisor:
Status: Graduated 2013 to 2015
Development of new chromatographic methods for the determination of plasma and urinary metanephrines

Kristýna Hanáková

Hanáková Kristýna's picture
Master training

Supervisor:
Status: Graduated 2011 to 2016
Study of modification of selected inhibitors of cyclin-dependent kinases targeted for biological transport

Marek Handl

marek.handl01@upol.cz's picture
Master training

Supervisor:
Status: Graduated 2013 to 2015
Distinctive effect of phenylpropanoids in ginger plant against acute T-lymphoblastic leukemia cells

Barbora Haroková

Haroková Barbora's picture
Master training

Supervisor:
Status: Graduated 2016 to 2018
Ribosomal stress and activation of p53

Richard Hekele

Hekele Richard's picture
Master training

Status: Graduated 2014 to 2016
Synthesis of trisubstituted derivatives of 4H-benzo triazole diazepin one on solid phase using an alpha-amino acid
This work is based on the solid-phase synthesis of trisubstituted 4H-benzotriazolodiazepinone derivatives utilizing alpha-amino acids. Used procedure comes from recently published findings of Fülöpová and Soural, and it is aimed at the synthesis of small library followed by biological screening (especially CNS activity).

Alžběta Herodesová

Herodesová Alžběta's picture
Master training

Status: Graduated 2015
Determination and clinical significance of genetic variants at risk of recurrent Crohn's disease
SVOČ 2015

Jiří Hodoň

Hodoň Jiří's picture
Master training

Supervisor:
Status: Graduated 2015 to 2017
Cytotoxic active triterpenes and their mechanism of action
The main goals of this work is to prepare a set of triterpenoid derivatives that have shown high selective cytotoxicity and synthesize biotinylated probes from them that may be used in pull down essays in order to try to find molecular targets. Also, new derivatives will be studied for their interference with cell cycle and DNA/RNA synthesis in cancer cells.

Michaela Holá

Holá Michaela MSc.'s picture
Master training

Supervisor:
Status: Graduated 2015 to 2017
Gallium-68 labelled RGD peptides evaluation for imaging of glioblastoma multiforme
The thesis is focused into critical evaluation of three different RGD peptides coupled with chelators to enable their radiolabelling with gallium-68. RGD peptides are accumulated in tumors expressing αvβ3 integrin (marker of angiogenesis). Such radiolabelled RGD peptides could be succesfully used to image tumors in vivo. Our study will include all appropriate stages of radiopharmaceutical testing i.e. optimalisation of lablelling conditions, radiochemical purity tests (HPLC, TLC), plasma stability assays, testing of stability in various chemical enviroments, octanol-water partition coefficient determination. Suitable derivatives will be passed to in vitro tests employing cell line U-87 MG (glioblastoma multiforme) to assess their ability to bind to tumor cells. Finally the appropriate candidates will be used for in vivo imaging of gliomas in mouse model. The imaging will be carried out in microPET/CT system and its results will be compared with ex vivo biodistribution study.

Miloš Hollan

Hollan Miloš's picture
Master training

Status: Graduated 2014 to 2016
Use hydroxylamines for determination of peroxide compounds

David Homola

Homola David's picture
Master training

Status: Graduated 2015 to 2016
Study photo dynamic and sono dynamic responses to tumor cell lines
SVOČ

Eva Hrušková

Hrušková Eva B.Sc.'s picture
Master training

Supervisor:
Status: Graduated 2010 to 2015
Testing the temperature uniformity of the thermocyclers using melting temperature of the same amplicon
The thesis is reviewing calibration methods of thermal cyclers and the experimental part is focused on the using of melting temperature of the same amplicon as a new approach of calibration.

Zlata Hušková

Hušková Zlata B.Sc.'s picture
Master training

Status: Graduated 2013 to 2015
Molecular mechanisms of plant steroids and study of their receptor interactions in cancer cells
The aim of the study is to find mechanisms of action of the plant steroids in human prostate cancer cells using cellular and molecular techniques indicated the possible involvement of steroid receptors in their action.

Romana Christová

Christová Romana's picture
Master training

Supervisor:
Status: Graduated 2017 to 2018
Radiolabelled siderophore derivatives for detection of cancer and infection

Kamila Jahodíková

Jahodíková Kamila's picture
Master training

Supervisor:
Status: Graduated 2014 to 2016
The role of Aurora-A kinase in the regulation of repair crossink-hedged DNA

Kateřina Janíková

katerina.janikova01@upol.cz's picture
Master training

Supervisor:
Status: Graduated 2014 to 2016
Utilization of Chan-Lam Cross-Coupling Reaction for derivatization of heterocyclic systems containing imidazole ring

Barbora Jenčíková

Jenčíková Barbora's picture
Master training

Supervisor:
Status: Graduated 2015 to 2017
Expression of opioid receptors and its influence on the treatment of gastrointestinal cancer patients

Pavlína Kalábová

Kalábová Pavlína's picture
Master training

Supervisor:
Status: Graduated 2014 to 2018
Proteomics of the hair

Barbora Koblihová

Koblihová Barbora's picture
Master training

Status: Graduated 2017 to 2019
Optimization of somatic mutation diagnostics in selected tumors with help of massive parallel sequencing

Lucie Kotková

Kotková Lucie's picture
Master training

Status: Graduated 2015 to 2017
Optimization of library preparation for NGS

Ondřej Kováč

Kováč Ondřej's picture
Master training

Supervisor:
Status: Graduated 2011 to 2016
Nitrogen analogs and striga lactones mimetics

Petra Králová

Králová Petra's picture
Master training

Status: Graduated 2014 to 2016
Solid-Phase Synthesis of Novel Morpholine/Thiomorpholine-3-Carboxylic Acid Derivatives
This diploma thesis deals with the preparation of novel morpholine/thiomorpholine-3-carboxylic acids derivatives with use of solid phase synthesis. The starting materials for the preparation of target compounds are four types of resin with acid-labile linkers, natural α-amino acids, sulfonyl chlorides, α-bromoacetophenones, carboxylic acids or their chlorides, trifluoroacetic acid and reducing agent (triethylsilane). The Theoretical section is devoted to the literature summarizing methods for the preparation of the target derivatives. In the Discussion part there are described issues concerning the preparation of the desired compounds. The Experimental section presents general procedures for the preparation and isolation of these derivatives followed by analytical data of the individual substances. The Conclusion part summarizes our results and their evaluation. In the last part, there are attached annexes of the prepared compounds.

Michal Kriegelstein

michal.kriegelstein01@upol.cz's picture
Master training

Supervisor:
Status: Graduated 2014 to 2016
Synthesis of some derivatives of 1-(1H-indazol-3-yl)-1H-benzo[d]imidazol-2(3H)-one substitued in positions 5 and 6

Patricia Kriegelsteinová

Kriegelsteinová Patricia's picture
Master training

Supervisor:
Status: Graduated 2016 to 2018
Synthesis of benzothiadiazepines using solid-phase synthesis

Pavel Lasák

Lasák Pavel's picture
Master training

Supervisor:
Status: Graduated 2014 to 2016
Synthesis of benzo[c]phenanthridine alkaloids analogues

Martin Liptay

Liptay Martin's picture
Master training

Supervisor:
Status: Graduated 2012 to 2016
Post-translational modifications of RNF168 E3 ubiquitin ligase

Kateřina Lónová

Lónová Kateřina's picture
Master training

Supervisor:
Status: Graduated 2020
Mechanisms of action of selected A3 adenosine receptor ligands

Zuzana Loubalová

Loubalová Zuzana's picture
Master training

Supervisor:
Status: Graduated 2014 to 2016
Cytogenetic changes in colorectal cancer patients
Master’s thesis is focused on cytogenetic changes in colorectal cancer patients. The student will propose and construct series of DNA probes suitable for fluorescence in situ hybridizations (FISH). By using this set will be possible to describe degree of deletions on q-arm of chromosome 18. Deletions of 18q involve DCC gene and are often find in colorectal cancer patients. The impact of this mutation is still discussed and the data are not consitent. As was newly found, in this region other 3 genes responsible for chomosome instability are located. Therfore is possible that for clinical imact would be important the the degree of the deletion.

Lucie Mádrová

Mádrová Lucie M.Sc's picture
Master training

Supervisor:
Status: Graduated 2013 to 2015
Usage of lipid profile for study of inherited metabolic disorders
The aim of the study is to optimize LCMS method with focus on polar lipids and their subsequent identification by fragmentation spectra and spectral trees. The final step will be the application of this method on biological samples, statistical evaluation of the data and metabolite identification based fragmentation patterns.

Markéta Machálková

Machálková Markéta's picture
Master training

Supervisor:
Status: Graduated 2013 to 2015
LC-MS analýza vybraných fosfatidylcholinů v lidské plazmě

Athanasios Markos

Markos Athanasios's picture
Master training

Status: Graduated 2014 to 2016
Synthesis of new derivatives of 3-hydroxyquinoline-4 (1H) -one

Júlia Martinková

Martinková Júlia's picture
Master training

Supervisor:
Status: Graduated 2016 to 2018
Forms of HPV infection in women at risk for cervical carcinoma

Kristýna Michalcová

Michalcová Kristýna's picture
Master training

Supervisor:
Status: Graduated 2015 to 2017
Epigenetic resolution monozygotic twins

Kamila Němcová

Němcová Kamila's picture
Master training

Supervisor:
Status: Graduated 2014 to 2016
Checking the integrity of the genome in cancer stem cells of prostate cancer

Eva Neubauerová

Neubauerová Eva's picture
Master training

Status: Graduated 2014 to 2016
Identification of molecular targets by proteomic methods

Martin Ondra

Ondra Martin M.Sc.'s picture
Master training

Status: Graduated 2015 to 2017
Cellular model of ribosomal stress
The theoretical part of the diploma thesis summarizes information about nucleolus, ribosomal biogenesis, Mdm2 and p53 dependent and independent ribosomal stress. In the experimental part, vectors expressing nucleolin (NCL) and fibrillarin (FBL) fused with green fluorescent protein at its N-terminus were created. Transfection of human U2OS cells with plasmids expressing GFP-NCL and subsequent selection resulted in a stable fluorescent- labeled nucleoline (U2OS-pEGFP-NCL) cell line. By transfecting the U2OS cell line with plasmids expressing GFP-FBL and subsequent selection, fluorescent-labeled fibrillarin cell line (U2OS-pEGFP-FBL) was created. For the validation of FBL and NCL as possible markers of ribosomal stress, selected cytostatics known to disturb ribosomal biogenesis have been applied to U2OS-pEGFP-FBL and U2OS-pEGFP-NCL cell lines. Double RNA fluorescent in situ hybridization on specific rRNA locus was performed after application of cytostatic. Cisplatin, oxaliplatin and actinomycin-D caused the breakdown of the nucleus integrity and the release of FBL and NCL from the nucleolus to the nucleus, whereas 5-fluorouracil did not affect the integrity of the nucleus and the localization of FBL and NCL. From the results of the double rRNA FISH was evident that cisplatin, oxaliplatin and actinomycin-D blocked transcription of 47S rRNA, but did not block processing of pre-rRNA. Application of 5-fluorouracil caused stopping pre-rRNA processing, but transcription of 47S rRNA was not affected. The U2OS-pEGFP-NCL cell line was transfected with siRNA-RPL11 to silence expression of RPL11. The RPL11 depletion did not cause the loss of integrity of the nucleus and did not trigger p53 dependent ribosomal stress. Cells transfected with siRNA-RPL11 have been identified to accumulate pre-rRNA precursors. The combination of localization of these fluorescently labeled proteins with the double rRNA FISH method for pre-rRNA specific regions has proven to be a suitable system for identifying substances that may cause ribosomal stress.

Jakub Ondrouch

Ondrouch Jakub B.Sc.'s picture
Master training

Status: Graduated 2015
miRNA expression profiling in oesophageal disease
The microarray technology can measure expression of thousands miRNAs in parallel. The main objective of the study is to profile miRNA expression in oesophageal disease to find specific biomarkers for better diagnosis and therapy using microarray technology.

Růžena Padrtová

Padrtová Růžena's picture
Master training

Supervisor:
Status: Graduated 2012 to 2014
Study of the synthesis and biological activity of novel derivatives of pyrazolo [1,5-a] pyrimidine as dual inhibitors of cyclin-dependent, and FLT-3 kinase

Barbora Pastorková

Pastorková Barbora B.Sc.'s picture
Master training

Status: Graduated 2013 to 2014
Identification of molecular targets of anticancer drugs by immuno-affinity purification
The aim of the study is to identify possible molecular targets of anti-tumour indazole derivatives by immuno-affinity purification and MS analysis.

Stanislava Pavlíková

Pavlíková Stanislava's picture
Master training

Supervisor:
Status: Graduated 2013 to 2015
Diagnosis of selected inherited metabolic disorders of newborn screening by HPLC-MS / MS

Natália Podhorská

Podhorská Natália's picture
Master training

Status: Graduated 2015 to 2017
Epigenetic changes detection in cancer cells
Aberrant regulation at epigenetic level can directly and/or indirectly influence the genetic integrity and gene expression pattern of cells, resulting in the development of various types of disorders, including cancer. The local hypermethylation of tumor-suppressor genes and the global hypomethylation of genomic DNA often occur in human cancers. Multiple DNA methylation changes in the cancer methylome could be associated with the acquisition of drug or ionizing radiation resistance. Cancer cell lines resistant to 2‘-deoxy 5-azacytidine (decitabine) and also radioresistant cells were prepared at our institute. Our specific aim is to assay different genome location for methylation status in resistant cancer cell lines. For this purpose, we plan to use bisulfite sequencing using multiparallel sequencing platform. Our long-term aim is to elucidate the mechanism of drug- and radioresistance in cancer cells.

Vendula Rašková

Rašková Vendula B.Sc.'s picture
Master training

Supervisor:
Status: Graduated 2013 to 2016
Proteomics of cervical fluid
The aim of the study is to find specific biomarkers for prediction of successful in vitro fertilization in cervical mucus.

Ivana Reiterová

Reiterová Ivana's picture
Master training

Supervisor:
Status: Graduated 2015 to 2017
Preparation of cell-based reporter assay for high-throughput screening of small molecules as potential receptor for advanced glycation endproducts inhibitors
Receptor for advanced glycation endproducts (RAGE) plays an important role in many pathological processes and thus represents potential for research and development of diagnostic and therapeutic strategies. Pyrimidine appears attractive as a template for the design of novel small-molecule RAGE inhibitors in terms of the structural simplicity and the chemical stability. The main goal of the study is to assess the contribution of RAGE in pyrimidine derivatives induced signaling response.

Helena Rezková

Rezková Helena's picture
Master training

Supervisor:
Status: Graduated 2013 to 2015
Metabolite profiling of tyrosine kinase inhibitors in the plasma of patients with chronic myeloid leukemia

Veronika Ručilová

Ručilová Veronika Ph.D.'s picture
Master training

Supervisor:
Status: Graduated 2009 to 2011
Preparation of bisheterocyclic derivatives of 3-hydroxy-4(1H)-quinolinone-7-carboxamides
The focus of the submitted Diploma thesis is the solid phase synthesis of new bisheterocyclic derivatives of 2-substituted-3-hydroxyquinolin-4(1H)-one-7-carboxamides. The first part of literature search summarizes the threee main points: synthesis, biological and fluorescence properties of known derivatives of hydroxyquinolones. The conclusion of the theoretical part is devoted to the synthesis of o-phenylenediamines which are used as a building bloks of nitrogen heterocycles. The experimental part is focused on the development and optimalization of the above mentioned precursores . There are also attempts to a cyclization of correspondent intermediate to benzimidazole-hydroxyquinolones.

Eliška Růžičková

Růžičková Eliška B.Sc.'s picture
Master training

Status: Graduated 2013 to 2014
Evaluation of biological activity of demethylation drugs
The aim of the study is to prepare resistant cell lines to 5-azacytidine and characterise multidrug resistance to epigenetic therapeutics.

Kateřina Sikorová

Sikorová Kateřina B.Sc.'s picture
Master training

Status: Graduated 2015
Quantification of methylation by pyrosequencing in hemato-onkological diseases
The aim of the study is quantification of DNA methylation in selected genes – by pyrosequencing method, in two related hemato-oncological disease: Myelodysplastic syndromes (MDS) and Acute myeloid leukemia (AML).

Jan Skácel

Skácel Jan's picture
Master training

Supervisor:
Status: Graduated 2017 to 2019
Analysis of changes in TCR repertoire and cellular immune response in peripheral blood of patiens treated with ipilimumab or nivolumab for malignancy

Hanuš Slavík

Slavík Hanuš M.Sc.'s picture
Master training

Status: Graduated 2012 to 2014
Effect of analgesia on circulating tumor cells levels in colorectal cancer patients

Dagmar Smitalová

Smitalová Dagmar's picture
Master training

Status: Graduated 2014 to 2015
Extracellular expression of microRNA in pulmonary sarcoidosis

Ivana Spurná

Spurná Ivana's picture
Master training

Supervisor:
Status: Graduated 2013 to 2014
Study catalyzed and nekatalyzovaných Click reaction with selected 5'-azido nucleosides

Jarmila Stanková

Stanková Jarmila's picture
Master training

Supervisor:
Status: Graduated 2014 to 2016
Proteomic profile of CCRF-CEM cell line treated by 5-fluorouracil

Marcela Suchomelová

Suchomelová Marcela's picture
Master training

Status: Graduated 2015 to 2017
Characterization of vancomycin-resistant entorococci of human and animal origin
The aim of the thesis is to mapp the prevalence, phenotype and genotype of vancomycin-resistant enterococci (VRE) isolated from clinical materials of patients hospitalized in Faculty Hospital Olomouc, Czech Republic and from poultry samples obtained from the Olomouc and South Moravian Region. Vysoká škola zdravotníctva a sociálnej práce sv. Alžbety v Bratislave (Obor Laboratórne vyšetrovacie metódy v zdravotníctve)

Karolína Světlíková

Světlíková Karolína's picture
Master training

Supervisor:
Status: Graduated 2014 to 2016
The analysis of NOD2/CARD15 polymorphisms in patients with accute myocardial infarction

Marek Šebrle

Šebrle Marek's picture
Master training

Supervisor:
Status: Graduated 2011 to 2017
Study on metathesis triterpenoids with exoskeletarnimi double bonds

Miroslav Škrobánek

Škrobánek Miroslav's picture
Master training

Supervisor:
Status: Graduated 2013 to 2014
Development of methods for the preparation of imidazo [4 ', 5': 4,5] benzo [1,2-e] [1,4] diazepin-9 (3H) -one using solid-phase

Dávid Švec

Švec Dávid's picture
Master training

Status: Graduated 2016 to 2019
Data-driven optimization of compound properties and exploration of a chemical space

Monika Tomanová

Tomanová Monika's picture
Master training

Supervisor:
Status: Graduated 2014 to 2016
Study of C4-arylation of 4-bromo-3,5-dinitro-1H-pyrazole via the Suzuki-Miyaura cross-coupling reaction

Eva Umlaufová

Umlaufová Eva's picture
Master training

Supervisor:
Status: Graduated 2016 to 2018
Preclinical evaluation of 68Ga-pyoverdines for Pseudomonas imaging

Radka Urbanovská

Urbanovská Radka's picture
Master training

Status: Graduated 2014 to 2015
The synthesis of pyrimidine derivatives as novel fluorescent labels

David Vanda

Vanda David's picture
Master training

Supervisor:
Status: Graduated 2011 to 2016
Syntéza derivátů purinu a deazapurinu a studium jejich biologických vlastností

Pavel Vepřík

Vepřík Pavel's picture
Master training

Status: Graduated 2015 to 2017
Synthesis of fluorescent labels derived from bordipyrromethenu

Zuzana Zálešáková

Zálešáková Zuzana's picture
Master training

Supervisor:
Status: Graduated 2015 to 2017
Testing and optimization of new non-destructive method for determining the activity of cytidine deaminase for use of various cell lines

Petra Zapletalová

Zapletalová Petra's picture
Master training

Supervisor:
Status: Graduated 2014 to 2016
Optimalization of intact protein mass spectrometry
The main task in this thesis is to introduce and optimize methods used in non covalent and intact protein mass spectrometry. It is expected that student will get a deep knowledge about this problematics and will get used to operate with premium grade mass spectrometers.

Marta Zbožínková

Zbožínková Marta B.Sc.'s picture
Master training

Status: Graduated 2015
3D culture for the development of anticancer drugs
The aim of the study is to develop high throughput-compatible multicellular spheroid cultures of cancer cells for the screening of potential anticancer agents.

Marie Zdráhalová

Zdráhalová Marie B.Sc.'s picture
Master training

Status: Graduated 2012 to 2014
A GC×GC-TOF analysis of the human urine metabolome
This work is dealing with GC×GC TOF MS metabolomic analysis of urine sample. Silylation agent is used for derivatization. Urine sample used for optimization is analyzed by different column sets with varied polarity, width of stationary phase and internal diameter of column. BPX5/SolGel-WAX, BPX5/SLB-IL59, BPX50/SolGel-WAX, BPX50/BPX5, SolGel-WAX/BPX5, SLB-IL59/BPX50 are columns used for analysis in this work and compared. Separation success is characterized by orthogonality. Column combination BPX5/SolGel-WAX was determined as the best. This combination is used for urine sample analysis of healthy children and patients with glutaric aciduria.

Pavel Zoufalý

Zoufalý Pavel's picture
Master training

Supervisor:
Status: Graduated 2014 to 2016
Exploraton of click reaction approach in triterpenoids containing propargyl substituent
The main aim of this work is to introduce propargyl substituent to a triterpenoid molecules and optimize its reactions with azides via Huisgen cycloaddition.