Vávrová Anna

Detection and characterization of bacteria producing beta-lactamases isolated from patients with leukemia

The aim of the thesis is the detection and characterization of bacteria producing beta-lactamases isolated from patients with leukemia. All isolates of enterobacteria will be tested for the presence of blaTEM, blaSHV and blaCTX-M genes (responsible for extended-spectrum beta-lactamase production) using PCR method and the eventual occurrence of genes enabling carbapenemases production (KPC, SME, IMI, VIM, IMP, OXA enzyme types etc.) will be assessed. For direct detection of extended-spectrum beta-lactamase (ESBL) producers, new real-time PCR assay will be performed.