Dear colleagues,
I would like to invite you to the lecture by Denisa Kroupová called "Optimizing SPR Analysis of Carbonic Anhydrase IX–Inhibitor Interactions: Challenges in Immobilization and Data Quality". The lecture will be on Wednesday November 5, 2025 at 9AM in the room 1.09 at IMTM.
Abstarct
Surface plasmon resonance (SPR) is a powerful technique for monitoring biomolecular interactions in real time. In this work, I focus on the application of SPR to study the interaction between carbonic anhydrase IX (CA IX) and selected inhibitors from ITMT Chemical Library. The presentation will briefly introduce the SPR principle but will primarily address the key experimental challenges related to CA IX immobilization, signal stability, and reproducibility. Emphasis will be placed on troubleshooting common artifacts and on optimization strategies aimed at improving data quality and interpretation.