Zdráhalová Marie B.Sc.

A GC×GC-TOF analysis of the human urine metabolome

This work is dealing with GC×GC TOF MS metabolomic analysis of urine sample. Silylation agent is used for derivatization. Urine sample used for optimization is analyzed by different column sets with varied polarity, width of stationary phase and internal diameter of column. BPX5/SolGel-WAX, BPX5/SLB-IL59, BPX50/SolGel-WAX, BPX50/BPX5, SolGel-WAX/BPX5, SLB-IL59/BPX50 are columns used for analysis in this work and compared. Separation success is characterized by orthogonality. Column combination BPX5/SolGel-WAX was determined as the best. This combination is used for urine sample analysis of healthy children and patients with glutaric aciduria.