The IMTM High-Throughput Screening (HTS) core facility is one of the largest and a state-of-the-art screening robotic platform in academia. Our goal is to accelerate discovery and development of new potential small molecule therapeutics as well as to identify and characterize novel therapeutic/biological targets. We have over 10 years of experience in running large-scaled screening campaigns on a fully automated and flexible robotic platform (HighResBiosolutions Ltd.) Our qualified and skilled team has expertise in a liquid handling, robotic automation, variety of readout technologies and big data analytics and is ready to assist and collaborate with you to accomplish your project requirements.
Our skills:
- Screening assays in 384 – or 1536 – well plate format
- Biochemical assays (e.g. protein – protein and protein – nucleic acid interactions, enzyme inhibition assays, receptor – ligand binding assays)
- Cell - based assays (e.g. cell proliferation assays, reporter gene assays for mapping the most significant signaling pathways and receptors)
- Cell types: immortalized cell lines (non cancer and cancer), primary cells, stem cells, senescent cells, 3D cell cultures)
- Various detection methods (e.g. absorbance, luminescence, fluorescence, fluorescence polarization)
- High content analysis (e.g. phenotypic assays, cell painting, cell cycle analysis, DNA damage/repair activity, cytoskeleton integrity, colocalization studies)
- Compound Libraries:
IMTM: Commercial compound library (over 3.000 compounds with known activity)
IMTM: Proprietary Library (over 15.000 unique small molecules)
IMTM: DrugLike Library (a collection of 100.000 small molecules with druglike properties)
HTS Screening Workflow
