Contakt

Das Viswanath M.Sc., Ph.D.

Phone: +420 585 632 243
Groups: LEM, Staff, Postdoctoral Fellow

Postdoctoral training

Three-dimensional culture of cancer cells for screening of potential anticancer agents .

Three-dimensional (3D) cultures of cancer cells are increasingly being recognized as physiologically-relevant in vitro tumor models. My research focused on the development of cost-effective 3D cell culture assays to produce monoculture or co-culture spheroids of malignant and non-malignant cell lines microtiter plates. The assays are performed using reporter and non-reporter cell lines, automated liquid handling and robotic systems, spectrophotometry, and high-content imaging.

Status: Graduated from 2013 to 2015.

About me

I am a life science scientist at the Institute of Molecular and Translational Medicine of Palacký University in Olomouc. I did my undergraduate and postgraduate studies in India at Sri Sathya Sai Institute of Higher Learning, followed by a Ph.D. at Victoria University of Wellington, Wellington, New Zealand on the therapeutic role of microtubule-targeting small molecules for neurodegenerative diseases. In 2015, I did an internship at the Mario Negri Institute for Pharmacological Research in Milan, Italy on biochemical and structural properties of tau protein in relation to its critical role in the pathology of frontotemporal dementia and Alzheimer’s disease. Currently, I continue to work on 3D cell cultures as physiologically relevant in vitro tumor models in cancer drug discovery and development. Recently, I have also started working on neurodegenerative proteinopathies with an objective to better understand the pathobiology of misfolding and aggregation and prion-like spreading of aggregates of tau and alpha-synuclein.

 

Current research lines

 

3D cell cultures, anti-cancer drugs, drug resistance

Tauopathies, tau misfolding and aggregation, tau spreading

 

Selected publications

 

  1. Gruner B#, Brynda J#, Das V#, Sicha V#, Stepankova J, Nekvinda J, Holub J, Pospisilova K, Fabry M, Pachtl P, Král V, Kugler M, Masek V, Medvedikova M, Matějková S, Nova A, Lišková B, Gurská S, Džubák P, Hajdúch M, Řezáčová P. Metallacarborane sulfamides: unconventional, specific, and highly selective inhibitors of carbonic anhydrase IX. J Med Chem. 2019 Sep 30. doi: 10.1021/acs.jmedchem.9b00945. (#equal contribution)

  2. J Řehulka, N Narendran, I Frydrych, P Džubák, JH Miller, M Hajdúch, and V Das. Peloruside A Induced Cell Death in Hypoxia Is p53 Dependent in HCT116 Colorectal Cancer Cells. Journal of Natural Products. 2018, 81:634-640.

  3. V Das, F Bruzzese, P Konečný, F Iannnelli, A Budillon, and M Hajdúch. Pathophysiologically-relevant in vitro tumor models for screening of anticancer compounds. Drug Discovery Today. 2015, 20:848-55.

  4. V Das, DA Sim, and JH Miller. Effect of taxoid and non-taxoid site microtubule-stabilizing agents on axonal transport of mitochondria in untransfected and ECFP-htau40-transfected rat cortical neurons in culture. Journal of Neuroscience Research. 2014, 92:1155-1166.

  5. V Das and JH Miller. Microtubule stabilization by peloruside A and paclitaxel rescues degenerating neurons from okadaic acid-induced tau phosphorylation. European Journal of Neuroscience. 2012, 35:1705-1717.

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